RFLP of 16S-ITS rDNA region to differentiate Lactobacilli at species level

被引：22

作者：

Yavuz, E

Gunes, H

Bulut, C

Harsa, S

Yenidunya, AF ^{[1
]}

机构：

[1] Izmir Inst Technol, Fac Sci, Dept Biol, TR-35430 Izmir, Turkey

[2] Izmir Inst Technol, Biotechnol & Bioengn Programme, TR-35430 Izmir, Turkey

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2004年 / 20卷 / 06期

关键词：

lactic acid bacteria; restriction fragment length polymorphism; similarity analysis;

D O I：

10.1023/B:WIBI.0000043151.09366.d7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and ToqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.

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收藏

页码：535 / 537

页数：3

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