Crystallization of an AMPA receptor binding domain without agonist:: importance of carbohydrate content and flash-cooling conditions

被引：2

作者：

Féthière, J

Andersson, A

Keinänen, K

Madden, DR

机构：

[1] Max Planck Inst Med Res, Ion Channel Struct Res Grp, D-69120 Heidelberg, Germany

[2] Univ Helsinki, Viikki Bioctr, Dept Biosci, Div Biochem, FIN-00014 Helsinki, Finland

[3] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444900014104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An AMPA-specific ionotropic glutamate receptor binding domain was overexpressed using the baculovirus system and purified by immunoaffinity and metal-affinity chromatography. Purified protein was enzymatically deglycosylated. Both glycosylated and deglycosylated proteins crystallized under the same conditions and in the same space group (P2). In both cases, it was observed that the use of MPD as a cryoprotectant induced a significant reduction in the unit-cell volume compared with glycerol or sucrose. For crystals of deglycosylated protein, cryoprotection with MPD also yielded a dramatic improvement in resolution.

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页码：1625 / 1629

页数：5

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