Antifungal Activity of Storage 2S Albumins from Seeds of the Invasive Weed Dandelion Taraxacum officinale Wigg.

被引：27

作者：

Odintsova, T. I. ^{[2
]}

Rogozhin, E. A. ^{[1
]}

Sklyar, I. V. ^{[1
]}

Musolyamov, A. K. ^{[1
]}

Kudryavtsev, A. M. ^{[2
]}

Pukhalsky, V. A. ^{[2
]}

Smirnov, A. N. ^{[3
]}

Grishin, E. V. ^{[1
]}

Egorov, T. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Lab Neuroreceptors & Neuroregulators, Moscow 117997, Russia

[2] Russian Acad Sci, Vavilov Inst Gen Genet, Lab Plant Genet, Moscow 117809, Russia

[3] Timiryazev Moscow Agr Acad, Phytopathol Lab, Moscow 127550, Russia

来源：

PROTEIN AND PEPTIDE LETTERS | 2010年 / 17卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

Taraxacum officinale Wigg; antifungal protein; storage protein; 2S albumin; CHEESEWEED MALVA-PARVIFLORA; ANTIMICROBIAL PEPTIDES; IN-VITRO; HOMOLOGOUS PROTEIN; POWDERY MILDEW; PLANT DEFENSE; FUNGAL GROWTH; LATE BLIGHT; RESISTANCE; BARLEY;

D O I：

10.2174/092986610790963591

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, we isolated and characterized novel antifungal proteins from seeds of dandelion (Taraxacum officinale Wigg.). We showed that they are represented by five isoforms, each consisting of two disulphide-bonded large and small subunits. One of them, To-A1 was studied in detail, including N-terminal amino acid sequencing of both subunits, and shown to display sequence homology with the sunflower 2S albumin. Using different assays we demonstrated that dandelion 2S albumins possess inhibitory activity against phytopathogenic fungi and the oomycete Phytophtora infestans at micromolar concentrations with various isoforms differing in their antifungal activity. Thus, 2S albumins of dandelion seeds represent a novel example of storage proteins with defense functions.

引用

页码：522 / 529

页数：8

共 45 条

[1] The antifungal properties of a 2S albumin-homologous protein from passion fruit seeds involve plasma membrane permeabilization and ultrastructural alterations in yeast cells [J].

Agizzio, Ana Paula ;

Da Cunha, Maura ;

Carvalho, Andre O. ;

Oliveira, Marco Antonio ;

Ribeiro, Suzanna F. F. ;

Gomes, Valdirene M. .

PLANT SCIENCE, 2006, 171 (04) :515-522

[2] A 2S albumin-homologous protein from passion fruit seeds inhibits the fungal growth and acidification of the medium by Fusarium oxysporum [J].

Agizzio, AP ;

Carvalho, AO ;

Ribeiro, SDF ;

Machado, OLT ;

Alves, EW ;

Okorokov, LA ;

Samarao, SS ;

Bloch, C ;

Prates, MV ;

Gomes, VM .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2003, 416 (02) :188-195

[3] Naturally occurring broad-spectrum powdery mildew resistance in a central American tomato accession is caused by loss of Mlo function [J].

Bai, Yuling ;

Pavan, Stefano ;

Zheng, Zheng ;

Zappel, Nana F. ;

Reinstaedler, Anja ;

Lotti, Concetta ;

De Giovanni, Claudio ;

Ricciardi, Luigi ;

Lindhout, Pim ;

Visser, Richard ;

Theres, Klaus ;

Panstruga, Ralph .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2008, 21 (01) :30-39

[4]

Broekaert WF, 1997, CRIT REV PLANT SCI, V16, P297, DOI 10.1080/713608148

[5] LECTINS, LECTIN GENES, AND THEIR ROLE IN PLANT DEFENSE [J].

CHRISPEELS, MJ ;

RAIKHEL, NV .

PLANT CELL, 1991, 3 (01) :1-9

[6] Purification and characterization of basic proteins with in vitro antifungal activity from seeds of cotton, Gossypium hirsutum [J].

Chung, RPT ;

Neumann, GM ;

Polya, GM .

PLANT SCIENCE, 1997, 127 (01) :1-16

[7] Plant pathogens and integrated defence responses to infection [J].

Dangl, JL ;

Jones, JDG .

NATURE, 2001, 411 (6839) :826-833

[8] Plant-derived antifungal proteins and peptides [J].

De Lucca, AJ ;

Cleveland, TE ;

Wedge, DE .

CANADIAN JOURNAL OF MICROBIOLOGY, 2005, 51 (12) :1001-1014

[9] Diversity of wheat anti-microbial peptides [J].

Egorov, TA ;

Odintsova, TI ;

Pukhalsky, VA ;

Grishin, EV .

PEPTIDES, 2005, 26 (11) :2064-2073

[10]

García-Olmedo F, 1998, BIOPOLYMERS, V47, P479, DOI 10.1002/(SICI)1097-0282(1998)47:6<479::AID-BIP6>3.0.CO

← 1 2 3 4 5 →