Evaluation of a PCR test for the diagnosis of Brucella ovis infection in semen samples from rams

被引：31

作者：

Manterola, L

Tejero-Garcés, A

Ficapal, A

Shopayeva, G

Blasco, JM

Marin, CM

López-Goñi, I ^{[1
]}

机构：

[1] Univ Navarra, Dept Microbiol, Pamplona 31008, Spain

[2] Generalitat Catalunya, Lab Sanitat Ramadera, Lleida, Spain

[3] Serv Invest Agr, Diputac Gen Aragon, Zaragoza, Spain

[4] Kazakh Natl Mexico Univ, Dept Infect Dis, Almaty, Kazakhstan

来源：

VETERINARY MICROBIOLOGY | 2003年 / 92卷 / 1-2期

关键词：

PCR; Brucella ovis; Brucellosis; sheep bacteria; diagnosis bacteria; POLYMERASE-CHAIN-REACTION; SELECTIVE MEDIA; MELITENSIS; MILK; DNA; IDENTIFICATION; CATTLE; ASSAY; SHEEP;

D O I：

10.1016/S0378-1135(02)00310-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sensitivity and specificity of a PCR assay with primers derived from the insertion sequence IS6501 was compared with that of bacteriological culture and serological tests for the diagnosis of Brucella ovis infection in rams. No amplifications were detected with DNAs from the strains phylogenetically related to Brucella and from the seven bacterial species considered as the main etiologic agents of epididymitis in rams. In addition, the specificity of the PCR was 100% when testing semen samples from Brucella-free rams. The comparison of the semen culture and PCR results from 192 semen samples tested, showed a proportion of agreement of 0.91 between both tests. The PCR-based test described has sensitivity similar to that of semen culture and could be used as a complementary test for the direct diagnosis of Brucella ovis in semen samples of rams. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：65 / 72

页数：8

共 19 条

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