First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase:: evidence for the mechanism of chain length determination

被引：30

作者：

Nicholson, TP ^{[1
]}

Winfield, C ^{[1
]}

Westcott, J ^{[1
]}

Crosby, J ^{[1
]}

Simpson, TJ ^{[1
]}

Cox, RJ ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

来源：

CHEMICAL COMMUNICATIONS | 2003年 / 06期

关键词：

D O I：

10.1039/b300847a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C-4 to C-8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

引用

页码：686 / 687

页数：2

共 12 条

[1]

Arthur C, 2002, CHEMBIOCHEM, V3, P253, DOI 10.1002/1439-7633(20020301)3:2/3<253::AID-CBIC253>3.0.CO

[2]

2-7

[3] Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis [J].

Bao, WL ;

Wendt-Pienkowski, E ;

Hutchinson, CR .

BIOCHEMISTRY, 1998, 37 (22) :8132-8138

[4] A chain initiation factor common to both modular and aromatic polyketide synthases [J].

Bisang, C ;

Long, PF ;

Cortés, J ;

Westcott, J ;

Crosby, J ;

Matharu, AL ;

Cox, RJ ;

Simpson, TJ ;

Staunton, J ;

Leadlay, PF .

NATURE, 1999, 401 (6752) :502-505

[5] Incubation of 6-methylsalicylic acid synthase with alternative starter units in the absence of NADPH and the identification of the resulting triaceticacid lactones [J].

Campuzano, IDG ;

Shoolingin-Jordan, PM .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1998, 26 (03) :S284-S284

[6] Streptomyces coelicolor phosphopantetheinyl transferase:: a promiscuous activator of polyketide and fatty acid synthase acyl carrier proteins [J].

Cox, RJ ;

Crosby, J ;

Daltrop, O ;

Glod, F ;

Jarzabek, ME ;

Nicholson, TP ;

Reed, M ;

Simpson, TJ ;

Smith, LH ;

Soulas, F ;

Szafranska, AE ;

Westcott, J .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 2002, (14) :1644-1649

[7] Genetic contributions to understanding polyketide synthases [J].

Hopwood, DA .

CHEMICAL REVIEWS, 1997, 97 (07) :2465-2497

[8] Structural control of polyketide formation in plant-specific polyketide synthases [J].

Jez, JM ;

Austin, MB ;

Ferrer, JL ;

Bowman, ME ;

Schröder, J ;

Noel, JP .

CHEMISTRY & BIOLOGY, 2000, 7 (12) :919-930

[9] MCAT is not required for in vitro polyketide synthesis in a minimal actinorhodin polyketide synthase from Streptomyces coelicolor [J].

Matharu, AL ;

Cox, RJ ;

Crosby, J ;

Byrom, KJ ;

Simpson, TJ .

CHEMISTRY & BIOLOGY, 1998, 5 (12) :699-711

[10] ENGINEERED BIOSYNTHESIS OF NOVEL POLYKETIDES [J].

MCDANIEL, R ;

EBERTKHOSLA, S ;

HOPWOOD, DA ;

KHOSLA, C .

SCIENCE, 1993, 262 (5139) :1546-1550

← 1 2 →