A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain

被引：3

作者：

Rockett, Rebecca J. ^{[1
,2
]}

Bialasiewicz, Seweryn ^{[1
,2
]}

Whiley, David M. ^{[1
,2
]}

Bletchly, Cheryl

Faux, Cassandra E. ^{[1
,2
]}

Lambert, Stephen B. ^{[1
,2
]}

Nimmo, Graeme R.

Nissen, Michael D. ^{[1
,2
]}

Sloots, Theo P. ^{[1
,2
]}

机构：

[1] Queensland Childrens Med Res Inst, Queensland Paediat Infect Dis Lab, Sir Albert Sakzewski Virus Res Ctr, Childrens Hlth Serv Dist, Brisbane, Qld, Australia

[2] Univ Queensland, Clin Med Virol Ctr, Brisbane, Qld 4072, Australia

来源：

VIRUSES-BASEL | 2009年 / 1卷 / 03期

关键词：

influenza; H1N1; real-time; PCR; detection;

D O I：

10.3390/v1031204

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.

引用

页码：1204 / 1208

页数：5

共 4 条

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