Assessment of screening methods for the identification of genetically modified potatoes in raw materials and finished products

被引：17

作者：

Jaccaud, E ^{[1
]}

Höhne, M ^{[1
]}

Meyer, R ^{[1
]}

机构：

[1] Nestec Ltd, Nestle Res Ctr, Dept Qual & Safety Assurance, CH-1000 Lausanne 26, Switzerland

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2003年 / 51卷 / 03期

关键词：

Solarium tuberosum; potato; GMO detection; polymerase chain reaction; nested PCR; CryIII A gene; potato leafroll virus; potato virus Y; patatin;

D O I：

10.1021/jf0208031

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Qualitative polymerase chain reaction methods for the detection of genetically modified potatoes have been investigated that can be used for screening purposes and identification of insect-resistant and virus-resistant potatoes in food. The presence of the nos terminator from Agrobacterium tumefaciens and the antibiotic marker gene nptl I (neomycin-phosphotransferase II) was demonstrated in three commercialized Bt-potato lines (Monsanto Co., St. Louis, MO, USA) and one noncommercial GM-potato product (high amylopectin starch, AVEBE, Veendam, The Netherlands) and allows for general screening in foods. For further identification, specific primers for the FMV promoter derived from the figwort mosaic virus, the CryIIIA gene (delta-endotoxin from Bacillus thuringiensis subsp. tenebrionis), potato leafroll virus replicase gene, and the potato virus Y coat protein gene, were designed. The methods described were successfully applied to processed potato raw materials (dehydrated potato powders and flakes), starch samples, and finished products.

引用

页码：550 / 557

页数：8

共 29 条

[1] Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products [J].

Anklam, E ;

Gadani, F ;

Heinze, P ;

Pijnenburg, H ;

Van Den Eede, G .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2002, 214 (01) :3-26

[2] NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5 [J].

BECK, E ;

LUDWIG, G ;

AUERSWALD, EA ;

REISS, B ;

SCHALLER, H .

GENE, 1982, 19 (03) :327-336

[3]

BRODMANN P, 1997, COMMUNICATION 0915

[4]

CERON J, 1995, APPL ENVIRON MICROB, V61, P3826

[5] HETEROLOGOUS ENCAPSIDATION OF POTATO VIRUS-Y STRAIN-O (PVYO) WITH THE TRANSGENIC COAT PROTEIN OF PVY STRAIN-N (PVYN) IN SOLANUM-TUBEROSUM CV BINTJE [J].

FARINELLI, L ;

MALNOE, P ;

COLLET, GF .

BIO-TECHNOLOGY, 1992, 10 (09) :1020-1025

[6]

Fischhoff D.A, 1996, U.S. Patent, Patent No. [5,495,071, 5495071]

[7] Detection of the starch modifying gbss-antisense construct in transgenic potatoes [J].

Hassan-Hauser, C ;

Mayer, W ;

Hortner, H .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1998, 206 (02) :83-87

[8]

JAMES C, 2001, GLOBAL STAT COMMERCI

[9]

JILKA JM, 1996, Patent No. 5589612

[10]

KECK PJ, 1994, SAFETY COMPOSITIONAL

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