Involvement of natural killer cells in patients with myelodysplastic syndrome carrying monosomy 7 revealed by the application of fluorescence in situ hybridization to cells collected by means of fluorescence-activated cell sorting

被引：32

作者：

Miura, I ^{[1
]}

Kobayashi, Y ^{[1
]}

Takahashi, N ^{[1
]}

Saitoh, K ^{[1
]}

Miura, AB ^{[1
]}

机构：

[1] Akita Univ, Sch Med, Dept Internal Med 3, Akita 0108543, Japan

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2000年 / 110卷 / 04期

关键词：

MDS; monosomy; 7; stem cell; lineage involvement; NK cell;

D O I：

10.1046/j.1365-2141.2000.02294.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Monosomy 7 is the most frequent chromosome abnormality among patients with secondary myelodysplastic syndrome (MDS). We used fluorescence in situ hybridization (FISH) and fluorescence-activated cell sorting (FACS) in order to clarify the lineage involvement. Four patients, three with de novo MDS and one with secondary MDS, were enrolled in this study. Monosomy 7 was observed in pluripotent stem cells (CD34(+)Thy-1(+)), and in B (CD34(+)CD19(+)) and T/natural killer (NK) progenitors (CD34(+)CD7(+)). The number of abnormal cells of B (CD19(+)) and T (CD3(+)) cells was below the cut-off value, but approximately 60% of the NK cells (CD3-CD56(+)) contained monosomy 7 in three of the patients.

引用

页码：876 / 879

页数：4

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