Selection of optical biosensors from chemisynthetic antibody libraries

被引：31

作者：

Jespers, L ^{[1
]}

Bonnert, TP ^{[1
]}

Winter, G ^{[1
]}

机构：

[1] MRC, Div Prot & Nucl Acid Chem, Lab Mol Biol, Cambridge CB2 2QH, England

来源：

PROTEIN ENGINEERING DESIGN & SELECTION | 2004年 / 17卷 / 10期

关键词：

antibody repertoire; chemisynthetic; optical biosensor;

D O I：

10.1093/protein/gzh083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method for creating antibodies with a fluorescent reporter integrated into the antigen-binding site. A reporter molecule was chemically linked to a hypervariable loop of an antibody repertoire displayed on phage, and this repertoire was selected for antigen binding. In one selected antibody, the fluorescence of the probe responded quantitatively to antigen binding. The method may have application for the engineering of homogeneous immunoassays.

引用

页码：709 / 713

页数：5

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