Structural insight into pre-B cell receptor function

被引：77

作者：

Bankovich, Alexander J.

Raunser, Stefan

Juo, Z. Sean

Walz, Thomas

Davis, Mark M.

Garcia, K. Christopher ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Program Immunol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Howard Hughes Med Inst, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

[5] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

[6] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

来源：

SCIENCE | 2007年 / 316卷 / 5822期

关键词：

D O I：

10.1126/science.1139412

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The pre-B cell receptor (pre-BCR) serves as a checkpoint in B cell development. In the 2.7 angstrom structure of a human pre-BCR Fab-like fragment, consisting of an antibody heavy chain (HC) paired with the surrogate light chain, the "unique regions" of VpreB and lambda 5 replace the complementarity-determining region 3 (CDR3) loop of an antibody light chain and appear to "probe" the HC CDR3, potentially influencing the selection of the antibody repertoire. Biochemical analysis indicates that the pre-BCR is impaired in its ability to recognize antigen, which, together with electron microscopic visualization of a pre-BCR dimer, suggests ligand-independent oligomerization as the likely signaling mechanism.

引用

页码：291 / 294

页数：4

共 28 条

[1] Standard conformations for the canonical structures of immunoglobulins
AlLazikani, B
Lesk, AM
Chothia, C
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (04) : 927 - 948
[2] BORK P, 1994, J MOL BIOL, V242, P309, DOI 10.1006/jmbi.1994.1582
[3] Interaction of murine precursor B cell receptor with stroma cells is controlled by the unique tail of λ5 and stroma cell-associated heparan sulfate
Bradl, H
Wittmann, J
Milius, D
Vettermann, C
Jäck, HM
[J]. JOURNAL OF IMMUNOLOGY, 2003, 171 (05) : 2338 - 2348
[4] The evolutionary and structural 'logic' of antigen receptor diversity
Davis, MM
[J]. SEMINARS IN IMMUNOLOGY, 2004, 16 (04) : 239 - 243
[5] DECKER DJ, 1991, J IMMUNOL, V146, P350
[6] Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering
Gauthier, L
Rossi, B
Roux, F
Termine, E
Schiff, C
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 13014 - 13019
[7] Cellular and genetic mechanisms of self tolerance and autoimmunity
Goodnow, CC
Sprent, J
Fazekas de St Groth, B
Vinuesa, CG
[J]. NATURE, 2005, 435 (7042) : 590 - 597
[8] IMMUNOGLOBULIN HEAVY-CHAIN AND BINDING-PROTEIN COMPLEXES ARE DISSOCIATED INVIVO BY LIGHT CHAIN ADDITION
HENDERSHOT, LM
[J]. JOURNAL OF CELL BIOLOGY, 1990, 111 (03) : 829 - 837
[9] Induction of pre-B cell proliferation after de novo synthesis of the pre-B cell receptor
Hess, J
Werner, A
Wirth, T
Melchers, F
Jäck, HM
Winkler, TH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) : 1745 - 1750
[10] THE EXPRESSION OF V-PRE-B/LAMBDA-5 SURROGATE LIGHT-CHAIN IN EARLY BONE-MARROW PRECURSOR B-CELLS OF NORMAL AND B-CELL-DEFICIENT MUTANT MICE
KARASUYAMA, H
ROLINK, A
SHINKAI, Y
YOUNG, F
ALT, FW
MELCHERS, F
[J]. CELL, 1994, 77 (01) : 133 - 143

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