False negative factor V Leiden assay following allogeneic stem cell transplant

被引：11

作者：

Crookston, KP ^{[1
]}

Henderson, R ^{[1
]}

Chandler, WL ^{[1
]}

机构：

[1] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1998年 / 100卷 / 03期

关键词：

activated protein C resistance; factor V Leiden; thrombophilia; thrombosis; transplantation;

D O I：

10.1046/j.1365-2141.1998.00585.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We report a case where a phenotypic test (an activity assay for activated protein C resistance) correctly indicated that the patient had an abnormality. whereas the initial genetic test (a PCR-based DNA assay used to detect the mutation in the FV gene) incorrectly indicated that the patient did not. The apparent false negative result of the DNA-based test was due to the use of peripheral blood leucocytes for DNA analysis. The patient had undergone a stem cell transplant several months before, and the leucocytes in her blood were derived from the stem cell donor, which lacked the FV defect.

引用

页码：600 / 602

页数：3

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