Diversity of selected hop cultivars detected by fluorescent AFLPs

被引：76

作者：

Hartl, L ^{[1
]}

Seefelder, S ^{[1
]}

机构：

[1] Bayer Landesanstalt Bodenkultur & Pflanzenbau, D-85354 Freising Weihenstephan, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 1998年 / 96卷 / 01期

关键词：

AFLP; fluorescence; hop; Humulus lupulus; genetic distance;

D O I：

10.1007/s001220050716

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The amplified fragment length polymorphism (AFLP) technique was used to assay eight hop cultivars. The application of fluorescent-labelled primers proved to be a valuable tool and substituted radiolabelling. Digestion with the enzymes EcoRI/MseI and amplification with primers having three selective bases at the 3'end resulted in distinct banding patterns for imaging with a fluorescent scanner. A total of 523 AFLP fragments derived from eight primer combinations were analysed. On average, 18 polymorphisms per combination were displayed. The Saazer "noble" hop cultivars 'Saazer', 'Tettnanger' and 'Spalter' could not be discriminated. The lowest genetic similarity (GS) between lines was computed for the bitter hops 'Hallertauer Magnum' and 'Wye Target': GS value of 0.89. The high level of genetic similarity of the analysed hop cultivars is discussed.

引用

页码：112 / 116

页数：5

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