Random transposon mutagenesis of Campylobacter jejuni

被引：22

作者：

Golden, NJ

Camilli, A

Acheson, DWK

机构：

[1] New England Med Ctr, Div Geog Med & Infect Dis, Boston, MA 02111 USA

[2] Tufts Univ, Dept Pathol & Immunol, Boston, MA 02111 USA

[3] Tufts Univ, Dept Mol Microbiol, Boston, MA 02111 USA

来源：

INFECTION AND IMMUNITY | 2000年 / 68卷 / 09期

关键词：

D O I：

10.1128/IAI.68.9.5450-5453.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. Insertions were random, as demonstrated by Southern blot analysis and insertional junction sequencing. We have demonstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni.

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页码：5450 / 5453

页数：4

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