Degradation of lysine in rice seeds:: Effect of calcium, ionic strength, S-adenosylmethionine and S-2-aminoethyl-L-cysteine on the lysine 2-oxoglutarate reductase-saccharopine dehydrogenase bifunctional enzyme

Lysine biosynthesis has been extensively studied and the regulatory enzymes characterized in some of the most important crop plants, however, much less is known about the lysine degradation pathway. Lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDN) have recently been partially purified and characterized from plants, and have been shown to exist as a single bifunctional polypeptide, We have further characterized these enzymes from rice endosperm in relation to Ca2+ and ionic strength modulation, Optimum pH values of 7.0 and 8.0 were obtained for LOR and SDH, respectively, The LOR domain of the polypeptide was modulated by Ca2+ and ionic strength, whereas the SDH domain was not, It would appear that the modulation by Ca2+ and ionic strength of LOR is a common feature among plant LOR enzymes, S-adenosylmethionine (SAM) did not produce any significant effect on either enzyme activity, indicating that it only plays a role in the regulation of lysine biosynthesis. The effect of S-2-aminoethyl-L-cysteine (AEC) as both a substrate and an inhibitor of LOR activity was also tested, AEC was shown to partially substitute for lysine as a substrate for LOR, but was also able to inhibit LOR activity, possibly competing with lysine at the active site. The higher K-m for AEC compared to lysine may reflect a lower binding affinity for AEC.