An in vitro assay for assessing the effects of growth factors on Nicotiana alata pollen tubes

被引:16
作者
Lush, WM [1 ]
Opat, AS [1 ]
Nie, F [1 ]
Clarke, AE [1 ]
机构
[1] Univ Melbourne, Sch Bot, Plant Cell Biol Res Ctr, Parkville, Vic 3052, Australia
来源
SEXUAL PLANT REPRODUCTION | 1997年 / 10卷 / 06期
基金
澳大利亚研究理事会;
关键词
bioassay; extracellular matrix; Nicotiana alata; pollen density; polyethylene glycol;
D O I
10.1007/s004970050109
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A new method for assessing the effects of test compounds on Nicotiana alata pollen tubes in culture is described. Pollen tubes grow from a cluster of grains placed beneath a thin layer of gelled medium in which test substances are incorporated and from which evaporation is prevented by a covering layer of oil. Pollen tubes can grow to 8 mm in length in 24 h, which corresponds to about 25% of the maximum growth rate in styles. Growth is non-destructively measured. The developmental stages reached by cultured tubes are similar to those of tubes growing in styles; growth changes from being reserve-dependent to reserve-independent, callose plugs form, and the nucleus of the generative cell divides. Because culture volumes are small (10-20 mu l per replicate), the effects of known concentrations of microgram quantities of compounds on the growth of pollen tubes can be tested.
引用
收藏
页码:351 / 357
页数:7
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