Histopathological and cytogenetic findings in benign, atypical and anaplastic human meningiomas:: a study of 60 tumors

Meningiomas may display benign (Grade I), atypical (Grade II) and anaplastic (Grade III) histopathological findings. The cytogenetic studies strongly suggest that secondary changes (moreover loss of chromosome 22) appear to be associated with more atypical features and with greater clinical aggressivity. We studied 60 tumors from 52 patients. Histopathological features such as nuclear pleomorphism, nucleolar prominence, mitosis, necrosis, cellular density, PCNA labeling index, and karyotype have been evaluated. Nuclear pleomorphism and nucleolar prominence showed a progressive increase in Grades I-III. Multifocal micronecrosis was considered a criterion of malignancy. A significant correlation was observed between PCNA-LI, mitotic index and grades. Complex karyotypes increased progressively: benign (34% of cases), atypical (45% of cases) and anaplastic (70% of cases). The most common numerical alterations were losses of chromosomes 10, 14, 18 and 22. The chromosomes most often involved in structural anomalies were: 1, 4, 7, 14 and 22. Telomeric associations was present in four cases and double minutes in two cases. Prognostic criteria for these tumors have been analyzed on the basis of these data.