N,N-diethylaniline as hydrogen donor for determination of hydrogen peroxide catalyzed by metalloporphyrins as enzyme mimetic of peroxidase

Various metalloporphyrins have been used as a catalyst instead of the peroxidase for the determination of hydrogen peroxide by formation of a dye from N,N-diethylaniline (DEA) and 4-aminoantipyrine. The difference of relative catalytic activity was investigated between enzyme and enzyme mimetics. FeT(4-TAP)P [5, 10, 15, 20-tetrakis(4-trimethyl-ammoniumphenyl)-21H, 23H-porphine] was shown as the best enzyme mimetic for horseradish peroxidase (HRP) among metalloporphyrins tested. 0 to 7.0 x 10(-5) mol/L hydrogen peroxide was determined with good accuracy and reproducibility, and giving recovery of 99.7-100.7%. DEA was certified as a sensitive color reagent in enzyme mimetic assay of hydrogen peroxide, with the apparent molar absorptivity for hydrogen peroxide was 1.37 x 10(4) L/mol.cm.