Separation and purification of strychnine from crude extract of Strychnos nux-vomica L. by high-speed countercurrent chromatography

被引：13

作者：

Miao, P ^{[1
]}

Cai, DC

Xiang, BR

An, DK

Ito, Y

机构：

[1] China Pharmaceut Univ, Instrument Ctr, Nanjing 210009, Peoples R China

[2] PLA Navy, Res Ctr Pharm, Shanghai 200083, Peoples R China

[3] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1998年 / 21卷 / 1-2期

关键词：

D O I：

10.1080/10826079808001945

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

High-speed countercurrent chromatography (CCC) was applied to the separation of strychnine and brucine from crude extract of Strychnos nux-vomica L. using a two-phase solvent system composed of chloroform/0.07 M sodium phosphate, 0.04 M citric buffer (pH 5.08) (1:1, v/v). Fractionated components were identified with authentic pure compounds on TLC and also analyzed by HPLC, LR and FTMS. The results showed that from 40 g of the seed powder, 48.2 mg of strychnine was purified at 99.9% purity (83.3% recovery) while 18.1 mg of brucine fraction was obtained after rechromatographed by preparative TLC improving the purity to 91.2%.

引用

页码：163 / 170

页数：8

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