A conserved role for kinesin-5 in plant mitosis

被引:74
作者
Bannigan, Alex
Scheible, Wolf-Ruediger
Lukowitz, Wolfgang
Fagerstrom, Carey
Wadsworth, Patricia
Somerville, Chris
Baskin, Tobias I. [1 ]
机构
[1] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA
[2] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany
[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[4] Carnegie Inst, Dept Plant Biol, Stanford, CA 94305 USA
关键词
Arabidopsis thaliana; AtKRP125; cortical microtubules; Eg5; gamma-tubulin; root morphology;
D O I
10.1242/jcs.009506
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The mitotic spindle of vascular plants is assembled and maintained by processes that remain poorly explored at a molecular level. Here, we report that AtKRP125c, one of four kinesin-5 motor proteins in arabidopsis, decorates microtubules throughout the cell cycle and appears to function in both interphase and mitosis. In a temperature-sensitive mutant, interphase cortical microtubules are disorganized at the restrictive temperature and mitotic spindles are massively disrupted, consistent with a defect in the stabilization of anti-parallel microtubules in the spindle midzone, as previously described in kinesin-5 mutants from animals and yeast. AtKRP125c introduced into mammalian epithelial cells by transfection decorates microtubules throughout the cell cycle but is unable to complement the loss of the endogenous kinesin-5 motor (Eg5). These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.
引用
收藏
页码:2819 / 2827
页数:9
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