Structure-function study of peroxidase-like G-quadruplex-hemin complexes

被引：108

作者：

Kong, De-Ming ^{[1
,2
]}

Yang, Wei ^{[2
]}

Wu, Jing ^{[2
]}

Li, Chen-Xi ^{[2
]}

Shen, Han-Xi ^{[2
]}

机构：

[1] Nankai Univ, Minist Educ, Key Lab Funct Polymer Mat, Tianjin 300071, Peoples R China

[2] Nankai Univ, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China

来源：

ANALYST | 2010年 / 135卷 / 02期

基金：

中国国家自然科学基金;

关键词：

INTRAMOLECULAR DNA QUADRUPLEXES; COLORIMETRIC DETECTION; TELOMERASE ACTIVITY; CIRCULAR-DICHROISM; CATALYTIC DNA; NUCLEIC-ACIDS; DNAZYME; APTAMER; BINDING; ION;

D O I：

10.1039/b920293e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The structure-function relationship of G-quadruplex-hemin complexes with peroxidase activity was studied by comparing peroxidase activity and circular dichroism (CD) spectra of 22 oligonucleotides with the sequence of d(G(2)T(n))(3)G(2), d(G(3)T(n))(3)G(3) (n = 1-4) and dG(3)T(i)G(3)T(j)G(3)T(k)G(3). According to the experimental results, some conclusions can be drawn, such as the addition of hemin may promote the conversion of some G-quadruplexes from antiparallel structures to parallel structures; the formation of G-quadruplexes is a crucial factor in determining the peroxidase activity of G-quadruplex-hemin complexes; and the complexes formed by hemin and parallel G-quadruplexes have much higher peroxidase activity than those formed by hemin and antiparallel G-quadruplexes.

引用

页码：321 / 326

页数：6

共 34 条

[1] Molecular biology - Making catalytic DNAs [J].

Breaker, RR .

SCIENCE, 2000, 290 (5499) :2095-2096

[2] A sequence-independent study of the influence of short loop lengths on the stability and topology of intramolecular DNA G-quadruplexes [J].

Bugaut, Anthony ;

Balasubramanian, Shankar .

BIOCHEMISTRY, 2008, 47 (02) :689-697

[3] OLIGONUCLEOTIDE INTERACTIONS .3. CIRCULAR DICHROISM STUDIES OF CONFORMATION OF DEOXYOLIGONUCLEOTIDES [J].

CANTOR, CR ;

WARSHAW, MM ;

SHAPIRO, H .

BIOPOLYMERS, 1970, 9 (09) :1059-&

[4] Fluorescence-based melting assays for studying quadruplex ligands [J].

De Cian, Anne ;

Guittat, Lionel ;

Kaiser, Markus ;

Sacca, Barbara ;

Amrane, Samir ;

Bourdoncle, Anne ;

Alberti, Patrizia ;

Teulade-Fichou, Marie-Paule ;

Lacroix, Laurent ;

Mergny, Jean-Louis .

METHODS, 2007, 42 (02) :183-195

[5] Highly effective colorimetric and visual detection of nucleic acids using an asymmetrically split peroxidase DNAzyme [J].

Deng, Minggang ;

Zhang, Dan ;

Zhou, Yangyang ;

Zhou, Xiang .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (39) :13095-13102

[6] A DNAzyme cascade for the amplified detection of Pb2+ ions or L-histidine [J].

Elbaz, Johann ;

Shlyahovsky, Bella ;

Willner, Itamar .

CHEMICAL COMMUNICATIONS, 2008, (13) :1569-1571

[7] Four-stranded nucleic acids: structure, function and targeting of G-quadruplexes [J].

Huppert, Julian Leon .

CHEMICAL SOCIETY REVIEWS, 2008, 37 (07) :1375-1384

[8] Immobilization of DNAzyme as a thermostable biocatalyst [J].

Ito, Y ;

Hasuda, H .

BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (01) :72-77

[9] A new method for the study of G-quadruplex ligands [J].

Kong, De-Ming ;

Wu, Jing ;

Ma, Yong-E ;

Shen, Han-Xi .

ANALYST, 2008, 133 (09) :1158-1160

[10] Peroxidase activity-structure relationship of the intermolecular four-stranded G-quadruplex-hemin complexes and their application in Hg2+ ion detection [J].

Kong, De-Ming ;

Wu, Jing ;

Wang, Na ;

Yang, Wei ;

Shen, Han-Xi .

TALANTA, 2009, 80 (02) :459-465

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