Solid state protein monolayers: Morphological, conformational, and functional properties

被引：8

作者：

Pompa, PP

Biasco, A

Frascerra, V

Calabi, F

Cingolani, R

Rinaldi, R

Verbeet, MP

de Waal, E

Canters, GW

机构：

[1] Univ Lecce, INFM,Dept Innovat Engn, Biomol Elect Div, Natl Nanotechnol Labs, I-73100 Lecce, Italy

[2] Leiden Univ, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands

来源：

JOURNAL OF CHEMICAL PHYSICS | 2004年 / 121卷 / 21期

关键词：

D O I：

10.1063/1.1828038

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have studied the morphological, conformational, and electron-transfer (ET) function of the metalloprotein azurin in the solid state, by a combination of physical investigation methods, namely atomic force microscopy, intrinsic fluorescence spectroscopy, and scanning tunneling microscopy. We demonstrate that a "solid state protein film" maintains its nativelike conformation and ET function, even after removal of the aqueous solvent. (C) 2004 American Institute of Physics.

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页码：10325 / 10328

页数：4

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