Mandibular reconstruction with transforming growth factor-β1

被引:23
作者
Sherris, DA
Murakami, CS
Larrabee, WF
Bruce, AG
机构
[1] Mayo Clin & Mayo Fdn, Dept Otorhinolaryngol, Div Facial Plast Surg, Rochester, MN 55905 USA
[2] Univ Washington, Dept Otolaryngol Head & Neck Surg, Seattle, WA 98195 USA
[3] Bristol Myers Squibb Co, Pharmaceut Res Inst, Seattle, WA 98121 USA
关键词
D O I
10.1097/00005537-199803000-00011
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Hypothesis: Transforming growth factor-beta 1 (TGF-beta 1) plus demineralized bone matrix (DBM) will reconstruct a critical mandibular defect devoid of periosteum in a canine model. Study Design: Randomized, blinded, placebo controlled, prospective animal pilot study. Methods: Canine critical mandibular defects devoid of periosteum were reconstructed with DBM (group 1, n = 3) and DBM plus TGF-beta 1 (250 mu g TGF-beta 1/g DBM) (group 2, n = 3). Radiologic, histologic, and biomechanical testing was performed on the test group and control group specimens at 12 weeks after implantation. Results: A palpable bone bridge was present in the group 2 subjects 5 to 6 weeks after implantation and was never present in the group 1 subjects. Radiologic and histologic examination at the time of harvest (12 weeks after implantation) demonstrated a solid bone bridge in the group 2 subjects and a fibrous union in the group 1 subjects. Group 2 specimens demonstrated failure in four-point bending testing at an average maximum moment of 9.9 +/- 2.2 N-m. This value was 9.4% of the maximum moment of the contralateral nonoperated side. Group 1 specimens were palpably flexible on plate removal and had a biomechanical strength of 0. The difference in strength between group 1 and group 2 was statistically significant (P < 0.02), supporting the hypothesis that the addition of TGF-beta 1 to the DBM carrier resulted in the formation of significantly stronger bone in the critical gap. Conclusion: The addition of TGF-beta 1 to DBM results in healing of a critical bone defect devoid of periosteum in a higher mammalian model.
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收藏
页码:368 / 372
页数:5
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