Gelatinolytic activity of matrix metalloproteinase-2 and-9 in oesophageal carcinoma;: a study using in situ zymography

被引：72

作者：

Koyama, H ^{[1
]}

Iwata, H ^{[1
]}

Kuwabara, Y ^{[1
]}

Iwase, H ^{[1
]}

Kobayashi, S ^{[1
]}

Fujii, Y ^{[1
]}

机构：

[1] Nagoya City Univ, Sch Med, Dept Surg 2, Mizuho Ku, Aichi 4678801, Japan

来源：

EUROPEAN JOURNAL OF CANCER | 2000年 / 36卷 / 16期

关键词：

matrix metalloproteinase-2; matrix metalloproteinase-9; in situ zymography; gelatinolytic activity; oesophageal cancer;

D O I：

10.1016/S0959-8049(00)00297-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

in this study, we investigated the activity of matrix metalloproteinase (MMP)-2 and -9 by gelatine zymography, immunostaining and in situ gelatine zymography in 30 oesophageal squamous-cell carcinomas. The gelatinolytic activity in situ was detected in all cases with different patterns of localisation. Significant gelatinolysis by stromal cells adjacent to tumour nests was found in 12 cases. Strong gelatinolytic activity appeared within the tumour nest itself in 13 cases. In the other 5 cases, both stromal cells and tumour cells showed the gelatinolytic activity. Gelatine zymography demonstrated a correlation between vascular invasion and activation of MMP-9. It also demonstrated a correlation between lymph node metastasis, lymphatic or vascular invasion and activation of MMP-2. These results suggest that MMPs play an important role in the invasion of oesophageal carcinoma. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：2164 / 2170

页数：7

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