High-performance liquid chromatographic determination of cotinine in urine in isocratic mode

被引:36
作者
Ceppa, F
El Jahiri, Y
Mayaudon, H
Dupuy, O
Burnat, P
机构
[1] Hop Instruct Armees Begin, Lab Biochim Toxicol & Pharmacol Clin, F-94160 St Mande, France
[2] Hop Instruct Armees Begin, Serv Endocrinol, F-94160 St Mande, France
来源
JOURNAL OF CHROMATOGRAPHY B | 2000年 / 746卷 / 02期
关键词
cotinine; nicotine; thiocyanates;
D O I
10.1016/S0378-4347(00)00306-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple procedure for the determination of cotinine, major metabolite of nicotine in urine, is described. The assay involved a liquid-liquid extraction with dichloromethane in alkaline environment. The extract was dried at ambient temperature under a gentle stream of nitrogen. The residue was dissolved in 300 mu l of mobile phase and 30 mu l 1 aliquot was injected via an automatic sampler into the liquid chromatograph and eluted with the mobile phase (10-9%, v/v methanol and acetonitrile, respectively in potassium dihydrogenphosphate buffer adjusted to pH 3.4) at a flow rate of 1 ml/min on a C-8 Symmetry cartridge column (5 mu m, 150 mmx3.9 mm, Waters) at 25 degrees C. The eluate was detected at 260 nm. Internal standard was 2-phenylimidazole. Sensitive and specific, this technique was performed to test urine of diabetic patients (smokers and non-smokers) admitted in an endocrinology service. Urinary cotinine seems to be a better marker of smoking status than thiocyanates. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:115 / 122
页数:8
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