Immunohistochemical analysis of cholecystokinin A receptor distribution in the rat pancreas

被引:9
作者
Kageyama, H
Kita, T
Horie, S
Takenoya, F
Funahashi, H
Kato, S
Hirayama, M
Lee, EY
Sakurai, J
Inoue, S
Shioda, S
机构
[1] Showa Univ, Sch Med, Dept Anat, Shinagawa Ku, Tokyo 1428555, Japan
[2] Tokai Univ, Dept Phys Educ, Kanagawa 2591292, Japan
[3] Tokai Univ, Res Inst Sports Med Sci, Kanagawa 2591292, Japan
[4] Hoshi Univ, Sch Pharm & Pharmaceut Sci, Dept Phys Educ, Tokyo 1428501, Japan
[5] Kyorusu Womens Univ, Fac Home Econ, Dept Nutr & Physiol, Tokyo 1018433, Japan
关键词
cholecystokinin A receptor; pancreatic islet; rat; immunohistochemistry; A cell; in situ hybridization;
D O I
10.1016/j.regpep.2004.08.033
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cholecystokinin (CCK) plays a major role in the regulation of pancreatic enzyme secretion based on its binding to the CCK-A receptor (CCK-AR). While CCK-AR is known to be expressed in rat islet B cells, the localization of CCK-AR in rat pancreatic A and D cells remains poorly understood. The aim of this study was to identify the localization of CCK-AR in rat pancreatic islets by means of double immunofluorescence straining with antibodies against CCK-AR, glucagon. insulin and somatostatin and with in situ hybridization to detect 9 its transcript. CCK-AR-like immunoreactive cells were found to overlap both with glucagon-like immunoreactive cells and insulin-like immunoreactive cells but not with somatostatin-like immunoreactive cells. An in situ hybridization study using a cRNA probe for CCK-AR revealed that CCK-AR mRNA was expressed in the center and periphery of die pancreatic islets. Further to this. immunofluoresence staining using anti-glucagon antibody was carried out after in situ hybridization using the CCK-.AR cRNA probe in order to identify CCK-AR mRNA expressing cells. CCK-AR mRNA exhibited a distribution pattern almost identical to that of glucagon-like immunoreactive cells. These results show clearly that CCK-AR exists not only in B but also in A cells of the rat pancreas, suggesting that CCK regulates the secretion of insulin and glucagon at least partly via CCK-AR. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:137 / 143
页数:7
相关论文
共 32 条
[1]   SECRETAGOGUE INDUCTION OF CELL-DIFFERENTIATION IN PANCREATIC ACINAR-CELLS INVITRO [J].
ARIAS, AE ;
BENDAYAN, M .
EXPERIMENTAL CELL RESEARCH, 1991, 195 (01) :199-206
[2]   EFFECTS OF ENDOGENOUS AND EXOGENOUS CHOLECYSTOKININ AND OF INFUSION WITH THE CHOLECYSTOKININ ANTAGONIST L-364,718 ON PANCREATIC AND GASTROINTESTINAL GROWTH [J].
AXELSON, J ;
HAKANSON, R ;
IHSE, I ;
LILJA, I ;
REHFELD, JF ;
SUNDLER, F .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1990, 25 (05) :471-480
[3]   Ontogeny and species differences in the pancreatic expression and localization of the CCKA receptors [J].
Bourassa, J ;
Lainé, J ;
Kruse, ML ;
Gagnon, MC ;
Calvo, É ;
Morisset, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 260 (03) :820-828
[4]   LITTLE OR NO EXPRESSION OF THE CHOLECYSTOKININ-A RECEPTOR GENE IN THE PANCREAS OF DIABETIC RATS (OTSUKA LONG-EVANS TOKUSHIMA FATTY=OLETF RATS) [J].
FUNAKOSHI, A ;
MIYASAKA, K ;
JIMI, A ;
KAWANAI, T ;
TAKATA, Y ;
KONO, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 199 (02) :482-488
[5]   Pancreatic endocrine dysfunction in rats not expressing the cholecystokinin-A receptor [J].
Funakoshi, A ;
Miyasaka, K ;
Kanai, S ;
Masuda, M ;
Yasunami, T ;
Nagai, T ;
Ikeda, S ;
Jimi, A ;
Kawanami, T ;
Kono, A .
PANCREAS, 1996, 12 (03) :230-236
[6]   PLASMA-GLUCAGON RESPONSES TO INSULIN-INDUCED HYPOGLYCEMIA AND ARGININE IN SPONTANEOUS NON-INSULIN-DEPENDENT DIABETES-MELLITUS (NIDDM) RATS, OTSUKA LONG-EVANS TOKUSHIMA FATTY (OLETF) STRAIN [J].
ISHIDA, K ;
MIZUNO, A ;
SANO, T ;
SHI, K ;
SHIMA, K .
ACTA ENDOCRINOLOGICA, 1993, 129 (06) :585-593
[7]   INHIBITION OF PANCREATIC ALPHA-AMINOISOBUTYRIC-ACID UPTAKE BY CHOLECYSTOKININ AND OTHER SECRETAGOGUES [J].
IWAMOTO, Y ;
WILLIAMS, JA .
AMERICAN JOURNAL OF PHYSIOLOGY, 1980, 238 (05) :G440-G444
[8]   INTERACTION OF CHOLECYSTOKININ WITH SPECIFIC MEMBRANE-RECEPTORS ON PANCREATIC ACINAR-CELLS [J].
JENSEN, RT ;
LEMP, GF ;
GARDNER, JD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (04) :2079-2083
[9]   INTERACTION OF CCK WITH PANCREATIC ACINAR-CELLS [J].
JENSEN, RT ;
WANK, SA ;
ROWLEY, WH ;
SATO, S ;
GARDNER, JD .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (10) :418-423
[10]  
KARLSSON S, 1992, SCAND J GASTROENTERO, V27, P161, DOI 10.3109/00365529208999942