Kinetic mechanism of NADH-enoyl-ACP reductase from Brassica napus

被引：22

作者：

Fawcett, T ^{[1
]}

Copse, CL ^{[1
]}

Simon, JW ^{[1
]}

Slabas, AR ^{[1
]}

机构：

[1] Univ Durham, Dept Biol Sci, Durham DH1 3LE, England

来源：

FEBS LETTERS | 2000年 / 484卷 / 02期

关键词：

enoyl reductase; crotonyl-ACP; crotonyl-coenzyme A; matrix-assisted laser desorption ionisation mass; spectrometry; Brassica napus;

D O I：

10.1016/S0014-5793(00)02128-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enoyl-ACP reductase, a component of fatty acid synthase, is a target for anti-microbial agents and herbicides. Here we demonstrate the kinetic mechanism to be a compulsory-order ternary complex with NADH binding before the acyl substrate. Matrix-assisted laser desorption ionisation mass spectrometry analysis of enzymatically and synthesised crotonyl-ACP substrate showed the former to contain a single acyl group, whereas the latter contained up to four additional crotonylations. The use of authentic crotonyl-ACP will be important in future kinetic and crystallographic studies. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：65 / 68

页数：4

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