Effects on the expression of GABAA receptor subunits by jujuboside A treatment in rat hippocampal neurons

被引：128

作者：

You, Zi-li ^{[1
]}

Xia, Qing ^{[1
]}

Liang, Fan-rong ^{[2
]}

Tang, Yi-jun ^{[1
]}

Xu, Cong-lun ^{[1
]}

Huang, Jian ^{[1
]}

Zhao, Ling ^{[2
]}

Zhang, Wen-zheng ^{[3
]}

He, Jia-jia ^{[3
]}

机构：

[1] Univ Elect Sci & Technol China, Sch Life Sci & Technol, Key Lab NeuroInformat, Minist Educ, Chengdu 610054, Peoples R China

[2] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Peoples R China

[3] Natl Key Lab Modern Commun, Chengdu 610041, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2010年 / 128卷 / 02期

关键词：

Semen Ziziphi Spinosae (SZS); Jujuboside A; Diazepam; Hippocampal neurons; RT-PCR; GABA(A) receptor subunit mRNAs; ZIZYPHI-SPINOSI-SEMEN; INDUCED SLEEPING BEHAVIORS; MESSENGER-RNAS; MASS-SPECTROMETRY; IN-VITRO; PHARMACOLOGY; DIAZEPAM; CONSTITUENTS; INVOLVEMENT; SANJOININE;

D O I：

10.1016/j.jep.2010.01.034

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Aim of study. To determine the effect of jujuboside A (JuA) in modulating the gamma-aminobutyric acid (GABA(A)) receptor subunits gene expression of hippocampal neurons at different terms in vitro Materials and methods Hippocampal neurons of rat were cultured in vitro, treated with JuA or diazepam (DZP). Then GABA(A) receptor mRNAs were evaluated by semi-quantitative RT-PCR Results JuA at the low dose of 41 mu M (about 0 05g/l) induced significant increase of GABA(A) receptor alpha 1, alpha 5, beta 2 subunit mRNAs in both 24 and 72 h treatments JuA at the high dose of 82 mu M (about 0 1g/l) significantly increased GABA(A) receptor alpha 1, alpha 5 subunit mRNA levels and decreased beta 2 subunit mRNA level at 24h treatment, and decreased GABA(A) receptor subunit alpha 1, beta 2 mRNAs expression at 72 h treatment DZP of 10 mu M significantly increased expression of GABA(A) receptor subunit alpha 1, alpha 5 and decreased expression of beta 2 at 24h treatment, and decreased alpha 1, alpha 5, beta 2 subunits gene expression at 72 h treatment Conclusion Differences in alterations in GABA(A) receptor subunit mRNAs expression following JuA and DZP treatments could help to explain the differences in the pharmacological action of the two drugs (C) 2010 Elsevier Ireland Ltd All rights reserved

引用

页码：419 / 423

页数：5

共 39 条

[1]

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn) [J].