Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes

被引:59
作者
Gutiérrez-Mateo, C
Wells, D
Benet, J
Sánchez-García, JF
Bermúdez, MG
Belil, I
Egozcue, J
Munné, S
Navarro, J
机构
[1] Univ Autonoma Barcelona, Dept Biol Cellular Fisiol & Immunol, Unitat Med, Fac Med, E-08193 Bellaterra, Spain
[2] St Barnabas Hosp, Inst Reprod Med & Sci, W Orange, NJ 07052 USA
[3] Inst Univ Dexeus, Serv Med Reprod, E-08017 Barcelona, Spain
关键词
aneuploidy; comparative genomic hybridization; first polar body; oocyte; preimplantation genetic diagnosis;
D O I
10.1093/humrep/deh367
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
BACKGROUND: Preimplantation Genetic Diagnosis (PGD) using FISH to analyze up to nine chromosomes to discard chromosomally abnormal embryos has resulted in an increase of pregnancy rates in certain groups of patients. However, the number of chromosomes that can be analyzed is a clear limitation. We evaluate the reliability of using comparative genomic hybridization (CGH) to detect the whole set of chromosomes, as an alternative to PGD using FISH. METHODS and RESULTS: We have analysed by CGH both, first polar bodies (1PBs) and metaphase II (MII) oocytes from 30 oocytes donated by 24 women. The aneuploidy rate was 48%. Considering two maternal age groups, a higher number of chromosome abnormalities were detected in the older group of oocytes (23% versus 75%, P<0.02). About 33% of the 1PB-MII oocyte doublets diagnosed as aneuploid by CGH would have been misdiagnosed as normal if FISH with nine chromosome probes had been used. CONCLUSION: We demonstrate the reliability of 1PB analysis by CGH, to detect almost any chromosome abnormality in oocytes as well as unbalanced segregations of maternal translocations in a time frame compatible with regular in vitro fertilization (IVF). The selection of euploid oocytes could help to increase implantation and pregnancy rates of patients undergoing IVF treatment.
引用
收藏
页码:2118 / 2125
页数:8
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