RNA interference targeting HBx suppresses tumor growth and enhances cisplatin chemosensitivity in human hepatocellular carcinoma

被引：75

作者：

Cheng, Alfred S. L.

Wong, Nathalie

Tse, Ada M. Y.

Chan, Kathy Y. Y.

Chan, Kai K.

Sung, Joseph J. Y.

Chan, Henry L. Y.

机构：

[1] Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Dept Anatom & Cellular Pathol, Shatin, Hong Kong, Peoples R China

来源：

CANCER LETTERS | 2007年 / 253卷 / 01期

关键词：

HBx; RNAi; cisplatin; chemosensitivity; hepatocellular carcinoma;

D O I：

10.1016/j.canlet.2007.01.004

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The X protein of hepatitis B virus (HBx) is often expressed in human hepatocellular carcinoma (HCC) but its role on tumor growth is not fully clarified. In this study, RNA interference was employed to knockdown HBx expression in Hep3B HCC cells, which naturally express carboxyl-end truncated form of HBx frequently found in HCC tissues. Specific knockdown of HBx strongly inhibited cell growth and tumorigenicity in xenograft model. HBx repression induced apoptosis in Hep3B cells and significantly increased cell sensitivity to cisplatin-induced apoptosis. These results suggest that RNA interference-mediated HBx suppression exerts potent anti-proliferative and chemosensitizing effects in human HCC. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：43 / 52

页数：10

共 38 条

[1]

Dual effects of hepatitis B virus X protein on the regulation of cell-cycle control depending on the status of cellular p53 [J].