Role of reactive oxygen species and phosphatidylinositol 3-kinase in cardiomyocyte differentiation of embryonic stem cells

被引：194

作者：

Sauer, H ^{[1
]}

Rahimi, C ^{[1
]}

Hescheler, J ^{[1
]}

Wartenberg, M ^{[1
]}

机构：

[1] Univ Cologne, Dept Neurophysiol, D-50931 Cologne, Germany

来源：

FEBS LETTERS | 2000年 / 476卷 / 03期

关键词：

reactive oxygen species; NADPH oxidase; phosphatidylinositol; 3-kinase; embryonic stem cell; embryoid body; cardiomyocyte differentiation;

D O I：

10.1016/S0014-5793(00)01747-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cardiotypic development in embryonic stem cell-derived embryoid bodies may be regulated by reactive oxygen species (ROS), ROS were generated by a NADPH oxidase-like enzyme which was transiently expressed during the time course of embryoid body development. Incubation with either H2O2 or menadione enhanced cardiomyogenesis, whereas the radical scavengers trolox, pyrrolidinedithiocarbamate and N-acetylcysteine exerted inhibitory effects. The phosphatidylinositol 3-kinase (PI3-kinase) inhibitors LY294002 and wortmannin abolished cardiac commitment and downregulated ROS in embryoid bodies. Coadministration of LY294002 with prooxidants resumed cardiomyocyte differentiation, indicating a role for PI-3-kinase in the regulation of the intracellular redox state. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：218 / 223

页数：6

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