A detailed protocol for the measurement of TGF-β1 in human blood samples

被引：39

作者：

Reinhold, D ^{[1
]}

Bank, U

Bühling, F

Junker, U

Kekow, J

Schleicher, E

Ansorge, S

机构：

[1] Univ Magdeburg, Inst Expt Internal Med, Dept Internal Med, D-39120 Magdeburg, Germany

[2] Univ Magdeburg, Inst Immunol, D-39120 Magdeburg, Germany

[3] Univ Jena, Inst Clin Immunol, D-07740 Jena, Germany

[4] Univ Magdeburg, Clin Rheumatol, D-39120 Magdeburg, Germany

[5] Univ Tubingen, Med Clin, D-72076 Tubingen, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1997年 / 209卷 / 02期

关键词：

D O I：

10.1016/S0022-1759(97)00160-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantification of the multifunctional cytokine Transforming Growth Factor-beta 1 (TGF-beta 1) in blood samples has aroused increasing interest in recent years, since an abnormal regulation of this cytokine appears to play a key role in the pathogenesis of different diseases; such as autoimmundiseases or malignant tumors. The measurement of TGF-beta 1 is complicated by a lot of problems concerning the collection, preparation and handling of blood samples, the platelet contamination, and the TGF-beta 1 activation procedure. Here, we recommend detailed instructions for measurement of TGF-beta 1 in blood plasma samples which should be followed to exclude the determination of false positive or negative results. (C) 1997 Elsevier Science B.V.

引用

页码：203 / 206

页数：4

共 7 条

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