Highly variable microsatellites in the California market squid Loligo opalescens

被引：14

作者：

Reichow, D ^{[1
]}

Smith, MJ ^{[1
]}

机构：

[1] Simon Fraser Univ, Inst Mol Biol & Biochem, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada

来源：

MARINE BIOTECHNOLOGY | 1999年 / 1卷 / 04期

关键词：

squid; Loligo; population structure; DNA markers; microsatellites; fisheries management;

D O I：

10.1007/PL00011795

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Attempts to study the genetic population structure of cephalopods are impeded by the low levels of genetic variation in these species. We have developed polymerase chain reaction (PCR) primers for six hypervariable microsatellite markers in order to analyze the molecular population structure in the Californian market squid Loligo opalescens. Each of these genomic loci has been cloned and fully sequenced. Here we report the sequence and properties of the six PCR primer sets for the amplification of hypervariable microsatellites. Heterozygosity levels in six squid samples from different locations are high for all loci tested.

引用

页码：403 / 406

页数：4

共 17 条

[1]

Ally JRR, 1978, CALIF FISH GAME FISH, V169, P113

[2]

AUGUSTYN CJ, 1988, MALACOLOGIA, V29, P215

[3]

Ausubel FM., 1994, Curr. Protoc. Mol. Biol

[4] GENE TIC VARIATION IN THE NERITIC SQUID LOLIGO-FORBESI (MYOPSIDA, LOLIGINIDAE) IN THE NORTHEAST ATLANTIC-OCEAN [J].

BRIERLEY, AS ;

THORPE, JP ;

PIERCE, GJ ;

CLARKE, MR ;

BOYLE, PR .

MARINE BIOLOGY, 1995, 122 (01) :79-86

[5] GENETIC-EVIDENCE OF POPULATION HETEROGENEITY AND CRYPTIC SPECIATION IN THE OMMASTREPHID SQUID MARTIALIA-HYADESI FROM THE PATAGONIAN SHELF AND ANTARCTIC POLAR FRONTAL ZONE [J].

BRIERLEY, AS ;

RODHOUSE, PG ;

THORPE, JP ;

CLARKE, MR .

MARINE BIOLOGY, 1993, 116 (04) :593-602

[6] MICROSATELLITES AND THEIR APPLICATION TO POPULATION GENETIC-STUDIES [J].

BRUFORD, MW ;

WAYNE, RK .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1993, 3 (06) :939-943

[7] BIOCHEMICAL GENETIC-STUDIES ON THE PATAGONIAN SQUID LOLIGO-GAHI DORBIGNY .1. ELECTROPHORETIC SURVEY OF GENETIC-VARIABILITY [J].

CARVALHO, GR ;

LONEY, KH .

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 1989, 126 (03) :231-241

[8] GENETIC DIVERSITY AND POPULATION DIFFERENTIATION OF THE SHORTFIN SQUID ILLEX-ARGENTINUS IN THE SOUTH-WEST ATLANTIC [J].

CARVALHO, GR ;

THOMPSON, A ;

STONER, AL .

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 1992, 158 (01) :105-121

[9] MICROSATELLITE VARIATION IN A SOCIAL INSECT [J].

CHOUDHARY, M ;

STRASSMANN, JE ;

SOLIS, CR ;

QUELLER, DC .

BIOCHEMICAL GENETICS, 1993, 31 (1-2) :87-96

[10]

CHRISTOFFERSON JP, 1978, FISH B CALIFORNIA, V169, P123

← 1 2 →