Reconstitution in vitro of the motile apparatus from the amoeboid sperm of Ascaris shows that filament assembly and bundling move membranes

被引:101
作者
Italiano, JE [1 ]
Roberts, TM [1 ]
Stewart, M [1 ]
Fontana, CA [1 ]
机构
[1] MRC,MOLEC BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND
关键词
D O I
10.1016/S0092-8674(00)80997-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed an in vitro motility system from Ascaris sperm, unique amoeboid cells that use filament arrays composed of major sperm protein (MSP) instead of an actin-based apparatus for locomotion. Addition of ATP to sperm extracts induces formation of fibers similar to 2 mu m in diameter. These fibers display the key features of the MSP cytoskeleton in vivo. Each fiber consists of a meshwork of MSP filaments and has at one end a vesicle derived from the plasma membrane at the leading edge of the cell. Fiber growth is due to filament assembly at the vesicle; thus, fiber elongation results in vesicle translocation, This in vitro system demonstrates directly that localized polymerization and bundling of filaments can move membranes acid provides a powerful assay for evaluating the molecular mechanism of amoeboid cell motility.
引用
收藏
页码:105 / 114
页数:10
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