Bioactivities and secondary structure of Mast Cell Degranulating (MCD) peptide analogs

被引:10
作者
Buku, A
Maulik, G
Hook, WA
机构
[1] CUNY Mt Sinai Sch Med, Dept Physiol & Biophys, New York, NY 10029 USA
[2] Univ Connecticut, Sch Med, Dept Surg, Farmington, CT 06030 USA
[3] Harvard Univ, Sch Med, Dept Radiat Oncol, Boston, MA 02215 USA
[4] NIDR, Clin Immunol Sect, Bethesda, MD 20892 USA
关键词
MCD peptide analogs; histamine; oxygen radicals; circular dichroism;
D O I
10.1016/S0196-9781(97)00253-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three analogs of Mast Cell Degranulating (MCD) peptide with C-terminal and one analog with N- and C-terminal deletions were synthesized and assayed for histamine releasing activity in mast cells. Des(20-22)-MCD and des(21)-MCD markedly decreased this activity. In des(16-17,21)-MCD this activity was completely abolished. By contrast, when the C-and N-termini were truncated in des(1-2,20-22)-MCD, the full activity of MCD peptide was restored. The possibility that these analogs trigger or inhibit oxygen radicals from neutrophils was examined in a cell-free system with a chemiluminescence assay. None of the above analogs exhibited inflammatory or anti-inflammatory activity. Changes in biological activities were correlated with structural changes, as seen by circular dichroism (CD) spectroscopy. (C) 1998 Elsevier Science Inc.
引用
收藏
页码:1 / 5
页数:5
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