Turkey microsatellite loci from an embryonic cDNA library

被引：22

作者：

Dranchak, PK ^{[1
]}

Chaves, LD ^{[1
]}

Rowe, JA ^{[1
]}

Reed, KM ^{[1
]}

机构：

[1] Univ Minnesota, Anim Biotechnol Ctr, St Paul, MN 55108 USA

来源：

POULTRY SCIENCE | 2003年 / 82卷 / 04期

关键词：

turkey; cDNA; expressed sequence tag; microsatellite; zoo blot;

D O I：

10.1093/ps/82.4.526

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Expressed sequence tags (EST) containing microsatellites have been used in the development of both genetic linkage maps and syntenic maps for various species and, thus, offer the advantage of being a convenient tool in comparative mapping studies. A turkey embryonic cDNA library was constructed and screened with (CA/TG)(15), (GA/CT)(15), (AGG)(10), and (AAAC)(7) probes for the development of polymorphic microsatellite markers. Sequences of 128 cDNA revealed 42 new loci containing microsatellites. BLAST nucleotide analysis demonstrated significant homology to known mammalian or avian coding regions for 15 of the turkey EST, five of which matched chicken transcripts. The remaining 27 EST represented novel sequences. Of the 42 new loci, 31 were polymorphic when tested on commercial turkey lines, including the founding individuals of a new resource population developed for genetic linkage mapping. Comparative mapping of these markers will provide new information toward the evolutionary divergence of turkey and chicken as well as other species.

引用

页码：526 / 531

页数：6

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