Simian-human immunodeficiency virus (SHIV) containing the nef long terminal repeat region of the highly virulent SIVsmmPBj14 causes PBj-Like activation of cultured resting peripheral blood mononuclear cells, but the chimera showed no increase in virulence

SIV(mmm)PBj14 is a highly pathogenic lentivirus which causes acute diarrhea, rash, massive lymphocyte proliferation predominantly in the gastrointestinal tract, and death within 7 to 14 days. In cell culture, the virus has mitogenic effects on resting macaque T lymphocytes. In contrast, SIV(mac)239 causes AIDS in rhesus macaques, generally within 2 years after inoculation. In a previous study, replacement of amino acid residues 17 and 18 of the Nef protein of SIV(mac)239 with the corresponding amino acid residues of the Nef protein of SIV(smm)PBj41 yielded a PBj-like virus that caused extensive activation of resting T lymphocytes in cultures and acute PBj-like disease when inoculated into pig-tailed macaques, This study suggested that nef played a major role in both processes. In this study, we replaced the nef/long terminal repeat (LTR) region of a nonpathogenic simian-human immunodeficiency virus (SHIV), SHIVPPc, with the corresponding region from SIV(smm)PBj14 and examined the biological properties of the resultant virus. Like SIV(smm)PBj14, SHIV(PPc)PBjnef caused massive stimulation of resting peripheral blood mononuclear cells (PBMC), which then produced virus in the absence of extraneous interleukin 2, However, when inoculated into macaques, the virus failed to replicate productively or cause disease, Thus, while these results confirmed that the nef/LTR region of SIV(smm)PBj14 played a major role in the activation of resting PBMC, duplication of the cellular activation process in macaques may require a further interaction between nef and the envelope glycoprotein of simian immunodeficiency virus because SHIV, containing the envelope of human immunodeficiency virus type 1, failed to cause activation in vivo.