Characterization of nociceptin-stimulated in situ [35S]GTPγS binding in comparison with opioid agonist-stimulated ones in brain regions of the mice

被引：34

作者：

Shimohira, I

Tokuyama, S

Himeno, A

Niwa, M

Ueda, H

机构：

[1] Nagasaki Univ, Sch Pharmaceut Sci, Dept Mol Pharmacol & Neurosci, Nagasaki 852, Japan

[2] Nagasaki Univ, Sch Med, Dept Pharmacol 1, Nagasaki 852, Japan

来源：

NEUROSCIENCE LETTERS | 1997年 / 237卷 / 2-3期

关键词：

nociceptin; ORL1; receptor; opioids; G protein; GTP gamma S binding; autoradiography;

D O I：

10.1016/S0304-3940(97)00807-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We studied the characterization of receptor-mediated G protein activity by nociceptin throughout brain regions, using in situ GTP gamma S binding autoradiography. Nociceptin-stimulated GTP gamma S binding was markedly observed in amygdala, hippocampal pyramidal cell layers, temporal and entorhinal cortex, infralimbic organ, anterior olfactory nucleus, and rostral part of thalamus. These nociceptin-stimulated activities were not affected by naloxone, naltrindol nor norbinaltorphimine which completely blocked mu-, delta- or kappa-opioid agonist-stimulated GTP gamma S binding, respectively. In addition, the distribution of nociceptin-stimulated activities throughout brain regions was found to be different from such opioid receptor-mediated ones. (C) 1997 Elsevier Science ireland Ltd.

引用

页码：113 / 116

页数：4

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