A quantitative diffraction-based sandwich immunoassay

被引：57

作者：

Goh, JB

Tam, PL

Loo, RW

Goh, MC

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

[2] Axela Biosensors Inc, Toronto, ON M5H 3W4, Canada

来源：

ANALYTICAL BIOCHEMISTRY | 2003年 / 313卷 / 02期

关键词：

biosensor; diffraction; immunoassay; microcontact printing; sandwich assay;

D O I：

10.1016/S0003-2697(02)00634-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It is shown that diffraction-based sensing can be enhanced for diagnostic purposes through the use of a secondary label. The limit of detection for anti-rabbit IgG was reduced more than 40-fold by using a gold-conjugated secondary antibody. The response to secondary antibody binding was linear for concentrations from 25 to 500 ng/ml of anti-rabbit IgG, suggesting that quantitative determinations can be readily done. Moreover, the binding of the secondary antibody was observed as soon as 1 min after its introduction to the surface-bound primary complex. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：262 / 266

页数：5

共 12 条

[1] Large-scale resonance amplification of optical sensing of volatile compounds with chemoresponsive visible-region diffraction gratings [J].