Real-time imaging of radioisotope labeled compounds in a living plant

被引:22
作者
Kanno, S. [1 ]
Rai, H. [1 ]
Ohya, T. [1 ]
Hayashi, Y. [1 ]
Tanoi, K. [1 ]
Nakanishi, T. M. [1 ]
机构
[1] Univ Tokyo, Dept Appl Biol Chem, Lab Radioisotope Plant Physiol, Grad Sch Agr & Life Sci ,Bunkyo Ku, Tokyo 1138657, Japan
关键词
D O I
10.1007/s10967-007-0625-z
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We developed a quantitative, real-time imaging system of labeled compounds in a living plant. The system was composed of CsI scintillator to convert beta-rays to visible light and an image intensifier unit (composed of GaAsP semiconductor and MCP; micro channel plate) to detect extremely weak light. When the sensitivity and resolution of the image of our system was compared with that of an imaging plate (IP), the sensitivity of our system (with 20 minutes) was higher than that of an IP, with similar quality to that of an IP. Using this system, the translocation of P-32 in a soybean plant tissue was shown in successive images.
引用
收藏
页码:565 / 570
页数:6
相关论文
共 7 条
[1]  
METTIVER G, 2004, NUCL I METH PHYS RES, V516, P544
[2]   Studies on the distribution, re-translocation and homeostasis of inorganic phosphate in barley leaves [J].
Mimura, T ;
Sakano, K ;
Shimmen, T .
PLANT CELL AND ENVIRONMENT, 1996, 19 (03) :311-320
[3]   Visualizing real time [11C]methionine translocation in Fe-sufficient and Fe-deficient barley using a Positron Emitting Tracer Imaging System (PETIS) [J].
Nakanishi, H ;
Bughio, N ;
Matsuhashi, S ;
Ishioka, NS ;
Uchida, H ;
Tsuji, A ;
Osa, A ;
Sekine, T ;
Kume, T ;
Mori, S .
JOURNAL OF EXPERIMENTAL BOTANY, 1999, 50 (334) :637-643
[4]  
NAKANISHI TM, IN PRESS J RADIOANAL
[5]  
Nakanishi Tomoko M., 2001, Radioisotopes, V50, P265, DOI 10.3769/radioisotopes.50.265
[6]  
Stumpf W. E., 1995, AUTDADIOGRAPHY CORRE
[7]   MEDICAL IMAGE MATCHING - A REVIEW WITH CLASSIFICATION [J].
VANDENELSEN, PA ;
POL, EJD ;
VIERGEVER, MA .
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1993, 12 (01) :26-39