Ultrafast high resolution separation of large DNA fragments by pulsed-field capillary electrophoresis

被引：36

作者：

Kim, Y ^{[1
]}

Morris, MD ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 01期

关键词：

pulsed-field capillary electrophoresis; hydroxyethyl cellulose; polyethylene oxide; restriction fragments;

D O I：

10.1002/elps.1150170125

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pulsed-field capillary electrophoresis (PFCE) in buffers containing ultradilute polymer solutions is used to separate long chain dsDNA in less than 4 min. Separations are shown to work with chain lengths below 10 kbp and greater than 1.5 Mbp. Several pulse protocols have been examined. If running time is to be minimized, a field inversion with higher peak amplitude in the forward direction than in the reverse, but with equal pulse durations, provides the best resolution. Other protocols can provide higher resolution. but only with longer running times.

引用

页码：152 / 160

页数：9

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