Determination of the prime electrostatic endothelial cell transplantation procedure for e-PTFE vascular prostheses

被引:7
作者
Bowlin, GL
Rittgers, SE
Schmidt, SP
Alexander, T
Sheffer, DB
Milsted, A
机构
[1] Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23298 USA
[2] Akron City Hosp, William H Falor Ctr Vasc Studies, Summa Hlth Syst, Akron, OH 44309 USA
[3] Univ Akron, Dept Biomed Engn, Akron, OH 44325 USA
[4] Akron City Hosp, Summa Hlth Syst, Dept Pathol, Akron, OH 44309 USA
[5] Univ Akron, Dept Biol, Akron, OH 44325 USA
关键词
electrostatic endothelial cell transplantation device; endothelial cell transplantation technique; vascular prosthesis preparation;
D O I
10.1177/096368970000900305
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The purpose of this study was to evaluate the extent of cellular adhesion (density and morphological maturation), cellular membrane damage, and cellular viability after an electrostatic transplantation of human umbilical vein endothelial cells (HUVECs) onto 6-cm segments of 4-mm I.D. e-PTFE (GORE-TEX(R)) vascular prostheses using a prototype electrostatic endothelial cell transplantation device (EECTD). The electrostatic transplantation parameters evaluated were the apparatus-applied voltage and transplantation time. By our definition, the combination of applied voltage and transplantation time that met the a priori criteria of: 1) maximum transplanted cellular viability, 2) maximum transplantation density, 3) maximum morphological maturation (degree of cellular flattening), and 4) minimal cellular membrane damage would be the prime transplantation procedure. The results of the experimentation indicated that the prime conditions for HUVEC transplantation were obtained when +1.0 V was applied for a transplantation time of 16 min. These conditions achieved an average viable graft surface coverage of 97.4 +/- 1.6% with an average transplantation density of 73,540 +/- 8,514 HUVECs/cm(2). Furthermore, the transplanted HUVECs were morphologically mature (flattened) with minimal apparent cellular membrane damage (lysis or pitting). The overall clinical significance of this study is that viable endothelial cell transplantation to synthetic vascular grafts can be accomplished at high cellular densities and morphological maturation in 16 min using the EECTD. With the promising in vitro transplantation results, the next logical investigations will include additional in vitro evaluations (cellular retention upon shear stress exposure and biochemical assays) followed by in vivo evaluations to examine thromboresistance and influence on intimal/anastomotic hyperplasia.
引用
收藏
页码:337 / 348
页数:12
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