Z-DNA-forming sites within the human beta-globin gene cluster

被引：23

作者：

Muller, V ^{[1
]}

Takeya, M ^{[1
]}

Brendel, S ^{[1
]}

Wittig, B ^{[1
]}

Rich, A ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 02期

关键词：

negative supercoiling; transcription rate; erythropoietic cells; topological domain;

D O I：

10.1073/pnas.93.2.780

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.

引用

页码：780 / 784

页数：5

共 16 条

[1] THE MOLECULAR GENETICS OF HUMAN-HEMOGLOBIN [J].