A moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells

被引：37

作者：

Teysset, L

Burns, JC

Shike, H

Sullivan, BL

Bucheton, A

Terzian, C

机构：

[1] CNRS,CTR GENET MOL,F-91198 GIF SUR YVETTE,FRANCE

[2] UNIV CALIF SAN DIEGO,SCH MED,DEPT PEDIAT,LA JOLLA,CA 92093

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 01期

关键词：

D O I：

10.1128/JVI.72.1.853-856.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gypsy element of Drosophila melanogaster is the first retrovirus identified so far in invertebrates. Previous data suggest that gypsy ENV-like ORF3 mediates viral infectivity. We have produced in the 293GP/LNhsp70lucL.3 human cell line a Moloney murine leukemia virus-based retroviral vector pseudotyped by the gypsy ENV-like protein. We have shown by immunostaining that the gypsy envelope protein is produced in 293GP/LNhsp70lucL.3 cells and that vector particles collected from these cells can infect Drosophila cells. Our results provide direct evidence that the infectious property of gypsy is due to its ORF3 gene product.

引用

收藏

页码：853 / 856

页数：4

相关论文

共 19 条

[1] Kinetics of retrovirus mediated gene transfer: The importance of intracellular half-life of retroviruses [J].

Andreadis, ST ;

Palsson, BO .

JOURNAL OF THEORETICAL BIOLOGY, 1996, 182 (01) :1-20

[2] VESICULAR STOMATITIS-VIRUS G GLYCOPROTEIN PSEUDOTYPED RETROVIRAL VECTORS - CONCENTRATION TO VERY HIGH-TITER AND EFFICIENT GENE-TRANSFER INTO MAMMALIAN AND NONMAMMALIAN CELLS [J].

BURNS, JC ;

FRIEDMANN, T ;

DRIEVER, W ;

BURRASCANO, M ;

YEE, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8033-8037

[3] RETROVIRAL RETARGETING BY ENVELOPES EXPRESSING AN N-TERMINAL BINDING DOMAIN [J].

COSSET, FL ;

MORLING, FJ ;

TAKEUCHI, Y ;

WEISS, RA ;

COLLINS, MKL ;

RUSSELL, SJ .

JOURNAL OF VIROLOGY, 1995, 69 (10) :6314-6322

[4] PROCESSING SPECIFICITY AND BIOSYNTHESIS OF THE DROSOPHILA-MELANOGASTER CONVERTASES DFURIN1, DFURIN1-CRR, DFURIN1-X, AND DFURIN2 [J].

DEBIE, I ;

SAVARIA, D ;

ROEBROEK, AJM ;

DAY, R ;

LAZURE, C ;

VANDEVEN, WJM ;

SEIDAH, NG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (03) :1020-1028

[5]

DECROLY E, 1994, J BIOL CHEM, V269, P12240

[6] STUDIES ON THE MECHANISM OF MEMBRANE-FUSION - SITE-SPECIFIC MUTAGENESIS OF THE HEMAGGLUTININ OF INFLUENZA-VIRUS [J].

GETHING, MJ ;

DOMS, RW ;

YORK, D ;

WHITE, J .

JOURNAL OF CELL BIOLOGY, 1986, 102 (01) :11-23

[7] COMPLETE NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF A DROSOPHILA TRANSPOSABLE GENETIC ELEMENT .297. [J].

INOUYE, S ;

YUKI, S ;

SAIGO, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 154 (02) :417-425

[8]

JORDAN TV, IN PRESS INSECT MOL

[9] RETROVIRUSES IN INVERTEBRATES - THE GYPSY RETROTRANSPOSON IS APPARENTLY AN INFECTIOUS RETROVIRUS OF DROSOPHILA-MELANOGASTER [J].

KIM, A ;

TERZIAN, C ;

SANTAMARIA, P ;

PELISSON, A ;

PRUDHOMME, N ;

BUCHETON, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1285-1289

[10] THE BACULOVIRUS-INTEGRATED RETROTRANSPOSON TED ENCODES GAG AND POL PROTEINS THAT ASSEMBLE INTO VIRUS-LIKE PARTICLES WITH REVERSE-TRANSCRIPTASE [J].

LERCH, RA ;

FRIESEN, PD .

JOURNAL OF VIROLOGY, 1992, 66 (03) :1590-1601