Cloning and characterization of the fur gene from Helicobacter pylori

被引：52

作者：

Bereswill, S ^{[1
]}

Lichte, F ^{[1
]}

Vey, T ^{[1
]}

Fassbinder, F ^{[1
]}

Kist, M ^{[1
]}

机构：

[1] Inst Med Microbiol & Hyg, Dept Microbiol & Hyg, D-79104 Freiburg, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 159卷 / 02期

关键词：

Helicobacter pylori; iron uptake; ferric uptake regulation; fur gene; Fur regulon;

D O I：

10.1016/S0378-1097(97)00566-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The fur homologue of Helicobacter pylori was isolated by screening a plasmid-based, genomic DNA library using the Fur titration assay (FURTA). The analysis of the DNA sequence revealed significant homology with Fur proteins from various other bacterial species. The highest degree of homology was observed for the Fur protein from Campylobacter jejuni. The H. pylori fur gene on a plasmid could partially complement the fur mutation in Escherichia coli strain H1681. The repressor activity depended on addition of iron to the medium indicating that iron acts as a co-repressor for the H. pylori protein similar to Fur from other bacteria: Comparison of Fur from H. pylori strain NCTC11638 with the recently published genomic DNA sequence of another strain (26695) confirmed the identity of the fur homologue and revealed that the fur locus is highly conserved in both strains. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

引用

页码：193 / 200

页数：8

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