Biotechnological applications of penicillin acylases:: state-of-the-art

被引：150

作者：

Arroyo, M ^{[1
]}

de la Mata, I ^{[1
]}

Acebal, C ^{[1
]}

Castillón, MP ^{[1
]}

机构：

[1] Univ Complutense Madrid, Dept Bioquim & Biol Mol 1, Fac Ciencias Biol, E-28040 Madrid, Spain

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2003年 / 60卷 / 05期

关键词：

D O I：

10.1007/s00253-002-1113-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This review describes the most recent developments in the biotechnological applications of penicillin acylases. This group of enzymes is involved mainly in the industrial production of 6-aminopenicillanic acid and the synthesis of semisynthetic beta-lactam antibiotics. In addition, penicillin acylases can also be employed in other useful biotrans formations, such as peptide synthesis and the resolution of racemic mixtures of chiral compounds. Particular emphasis is placed on advances in detection of new enzyme specificities towards other natural penicillins, enzyme immobilization, and optimization of enzyme-catalyzed hydrolysis and synthesis in the presence of organic solvents.

引用

页码：507 / 514

页数：8

共 100 条

[21] Cross-linked aggregates of penicillin acylase:: robust catalysts for the synthesis of β-lactam antibiotics [J].

Cao, L ;

van Langen, LM ;

van Rantwijk, F ;

Sheldon, RA .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 11 (4-6) :665-670

[22]

CAO L, 2000, Patent No. 108887

[23] Cross-linked enzyme aggregates: A simple and effective method for the immobilization of penicillin acylase [J].

Cao, LQ ;

van Rantwijk, F ;

Sheldon, RA .

ORGANIC LETTERS, 2000, 2 (10) :1361-1364

[24] Enzymatic resolution of alpha-alkyl beta-amino acids using immobilized penicillin G acylase [J].

Cardillo, G ;

Tolomelli, A ;

Tomasini, C .

JOURNAL OF ORGANIC CHEMISTRY, 1996, 61 (24) :8651-8654

[25] An approach for enhancing heterologous production of Providencia rettgeri penicillin acylase in Escherichia coli [J].

Chou, CP ;

Wang, WC ;

Lin, MI .

BIOTECHNOLOGY PROGRESS, 2000, 16 (03) :315-318

[26] Genetic strategies to enhance penicillin acylase production in Escherichia coli [J].

Chou, CP ;

Lin, WJ ;

Kuo, BY ;

Yu, CC .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (10) :766-773

[27]

De Vroom E, 1999, CHIM OGGI, V17, P65

[28]

de Vroom E., 1997, PCT Int. Pat., Patent No. [WO9704086, 9704086]

[29] Small bugs, big business: The economic power of the microbe [J].

Demain, AL .

BIOTECHNOLOGY ADVANCES, 2000, 18 (06) :499-514

[30] MOLECULAR-BIOLOGY OF BETA-LACTAM ACYLASES [J].

DESHPANDE, BS ;

AMBEDKAR, SS ;

SUDHAKARAN, VK ;

SHEWALE, JG .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1994, 10 (02) :129-138

← 1 2 3 4 5 6 7 8 9 10 →