The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM)

被引：27

作者：

Funaki, K ^{[1
]}

Nagata, A ^{[1
]}

Akimoto, Y ^{[1
]}

Shimada, K ^{[1
]}

Ito, K ^{[1
]}

Yamamoto, K ^{[1
]}

机构：

[1] Chiba Univ, Dept Biol, Inage Ku, Chiba 2638522, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2004年 / 45卷 / 09期

关键词：

2,3-butanedione monoxime (BDM); Chara corallina; cytoplasmic streaming; in vitro motility; plant myosin;

D O I：

10.1093/pcp/pch154

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We studied the effects of 2,3-butanedione monoxime (BDM) on the cytoplasmic streaming of Chara corallina and on the motility of myosin prepared from the same plant to examine whether this reagent really affects the plant class XI myosin. It was found that BDM inhibited both cytoplasmic streaming and the motility of myosin at a very similar concentration range (10-100 mM). BDM introduced directly into tonoplast-free cells also inhibited cytoplasmic streaming. These results suggested that effect of BDM on cytoplasmic streaming was exerted through myosin and not through ion channels at least in Chara corallina, though a very high concentration of BDM was required.

引用

页码：1342 / 1345

页数：4

共 32 条

[1]

Allen TJA, 1998, J PHYSIOL-LONDON, V508, P1

[2] Some motile properties of fast characean myosin [J].