Urinary electrophoretic profiles from chronic fatigue syndrome and chronic fatigue syndrome/fibromyalgia patients: a pilot study for achieving their normalization

被引：11

作者：

Casado, B

Zanone, C

Annovazzi, L

Iadarola, P

Whalen, G

Baraniuk, JN

机构：

[1] Univ Pavia, Dept Biochem A Castellani, I-27100 Pavia, Italy

[2] Georgetown Univ, Dept Med, Div Rheumatol Allergy & Immunol, Washington, DC 20057 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 814卷 / 01期

关键词：

chronic fatigue syndrome; fibromyalgia;

D O I：

10.1016/j.jchromb.2004.09.056

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim of our study was to determine if there were distinct, disease-related patterns of urinary analytes in chronic fatigue syndrome (CFS) and chronic fatigue syndrome/fibromyalgia (CFS/FM) compared to normal controls (NC). Urine was collected from these subjects for two consecutive 24 h periods and aliquots were submitted to micellar electrokinetic chromatography (MEKC). To compensate for the differences in peak migration times, these were normalized from the 35 min duration of run to a 100-point scale, and each peak was assigned its normalized time measure. Peak heights were also normalized by dividing the mAU by that of the internal standard (creatinine) and multiplying by 100. MEKC with normalization for peak height and migration time generated comparable results within each of the patient groups. CFS/FM and CFS had significant differences in peaks compared to NC that may be of significance as biomarkers of illnesses. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：43 / 51

页数：9

共 47 条

[1]

Alfazema LN, 2000, ELECTROPHORESIS, V21, P2503

[2] Micellar electrokinetic capillary chromatography (MECC) of UV-absorbing constituents in normal urine: A chemometric optimisation of the separation [J].

Alfazema, LN ;

Hows, MEP ;

Howells, S ;

Perrett, D .

ELECTROPHORESIS, 1997, 18 (10) :1847-1856

[3] Sensitive and high-throughput analyses of purine metabolites by dynamic pH junction multiplexed capillary electrophoresis: A new tool for metabolomic studies [J].

Britz-McKibbin, P ;

Nishioka, T ;

Terabe, S .

ANALYTICAL SCIENCES, 2003, 19 (01) :99-104

[4] COMPARISON OF URINARY-EXCRETION OF UV-ABSORBING CONSTITUENTS IN HEALTHY-SUBJECTS AND PATIENTS WITH RHEUMATOID-ARTHRITIS USING ANALYTICAL ISOTACHOPHORESIS [J].

CLARK, PMS ;

KRICKA, LJ ;

WHITEHEAD, TP .

JOURNAL OF CHROMATOGRAPHY, 1980, 181 (3-4) :347-354

[5]

Elgstoen KBP, 2001, J CHROMATOGR A, V914, P265

[6] Capillary electrophoresis for diagnosis of metabolic disease [J].

Elgstoen, KBP ;

Jellum, E .

ELECTROPHORESIS, 1997, 18 (10) :1857-1860

[7]

Ernberg M, 2000, J OROFAC PAIN, V14, P37

[8] THE CHRONIC FATIGUE SYNDROME - A COMPREHENSIVE APPROACH TO ITS DEFINITION AND STUDY [J].

FUKUDA, K ;

STRAUS, SE ;

HICKIE, I ;

SHARPE, MC ;

DOBBINS, JG ;

KOMAROFF, A ;

SCHLUEDERBERG, A ;

JONES, JF ;

LLOYD, AR ;

WESSELY, S ;

GANTZ, NM ;

HOLMES, GP ;

BUCHWALD, D ;

ABBEY, S ;

REST, J ;

LEVY, JA ;

JOLSON, H ;

PETERSON, DL ;

VERCOULEN, JHMM ;

TIRELLI, U ;

EVENGARD, B ;

NATELSON, BH ;

STEELE, L ;

REYES, M ;

REEVES, WC .

ANNALS OF INTERNAL MEDICINE, 1994, 121 (12) :953-959

[9] Chronic multisymptom illness affecting Air Force veterans of the Gulf War [J].

Fukuda, K ;

Nisenbaum, R ;

Stewart, G ;

Thompson, WW ;

Robin, L ;

Washko, RM ;

Noah, DL ;

Barrett, DH ;

Randall, B ;

Herwaldt, BL ;

Mawle, AC ;

Reeves, WC .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1998, 280 (11) :981-988

[10] Perception of noxious and innocuous heat stimulation among healthy women and women with fibromyalgia: association with mood, somatic focus, and catastrophizing [J].

Geisser, ME ;

Casey, KL ;

Brucksch, CB ;

Ribbens, CM ;

Appleton, BB ;

Crofford, LJ .

PAIN, 2003, 102 (03) :243-250

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