On-chip handling of solutions and electrochemiluminescence detection of amino acids

被引：33

作者：

Hosono, Hiroki ^{[1
]}

Satoh, Wataru ^{[1
]}

Fukuda, Junji ^{[1
]}

Suzuki, Hiroaki ^{[1
]}

机构：

[1] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2007年 / 122卷 / 02期

基金：

日本学术振兴会;

关键词：

microfluidic transport systems electrowetting; electrochemiluminescence; tris(2,2-bipyridyl)rutheniuin(II); amino acids;

D O I：

10.1016/j.snb.2006.06.030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-chip determination of amino acids was conducted by electrochemiluminescence (ECL) following microfluidic transport, mixing of solutions, and generation of ECL. The fabricated system consisted of a polydimethylsilixane (PDMS) substrate with a flow channel structure and three-electrode systems formed on a glass substrate that resulted in the microfluidic transport and the ECL. An amino acid solution and a reagent solution containing tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) were transported by electrowetting by applying a negative potential to gold working electrodes formed along the flow channels. The two solutions were then mixed in the mixing channel using an additional gold working electrode formed between the electrodes for transport. ECL was generated by applying a potential to a platinum working electrode formed in the mixing channel. The ECL from L-proline, L-lysine, L-leucine, L-valine, and L-histidine was examined. Clear ECL was observed, and its intensity depended on the concentration of the amino acids. By increasing the pH by mixing solutions and generating the oxidized form, Ru(bpy)(3), on the chip, the detection sensitivity could be improved. L-proline showed the strongest ECL, and concentrations of the nM order could be detected. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：542 / 548

页数：7

共 24 条

[1] Microfluidic immunosensor systems [J].

Bange, A ;

Halsall, HB ;

Heineman, WR .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (12) :2488-2503

[2]

BLACKBURN GF, 1991, CLIN CHEM, V37, P1534

[3] ROLE OF ELECTRON-DONATING WITHDRAWING CHARACTER, PH, AND STOICHIOMETRY ON THE CHEMILUMINESCENT REACTION OF TRIS(2,2'-BIPYRIDYL)RUTHENIUM(III) WITH AMINO-ACIDS [J].

BRUNE, SN ;

BOBBITT, DR .

ANALYTICAL CHEMISTRY, 1992, 64 (02) :166-170

[4] EFFECT OF PH ON THE REACTION OF TRIS(2,2'-BIPYRIDYL)RUTHENIUM(III) WITH AMINO-ACIDS - IMPLICATIONS FOR THEIR DETECTION [J].

BRUNE, SN ;

BOBBITT, DR .

TALANTA, 1991, 38 (04) :419-424

[5] CHEMILUMINESCENCE DETECTION USING REGENERABLE TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) IMMOBILIZED IN NAFION [J].

DOWNEY, TM ;

NIEMAN, TA .

ANALYTICAL CHEMISTRY, 1992, 64 (03) :261-268

[6] TOWARDS INTEGRATED MICROLIQUID HANDLING SYSTEMS [J].

ELWENSPOEK, M ;

LAMMERINK, TSJ ;

MIYAKE, R ;

FLUITMAN, JHJ .

JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 1994, 4 (04) :227-245

[7] Integrated microfluidic devices [J].

Erickson, D ;

Li, DQ .

ANALYTICA CHIMICA ACTA, 2004, 507 (01) :11-26

[8] On-chip generation and detection of electrochemiluminescence [J].

Fiaccabrino, GC ;

de Rooij, NF ;

Koudelka-Hep, M .

ANALYTICA CHIMICA ACTA, 1998, 359 (03) :263-267

[9] Lab-on-a-chip: A revolution in biological and medical sciences. [J].

Figeys, D ;

Pinto, D .

ANALYTICAL CHEMISTRY, 2000, 72 (09) :330A-335A

[10] Analytical applications of tris(2,2′-bipyridyl)ruthenium(III) as a chemiluminescent reagent [J].

Gerardi, RD ;

Barnett, NW ;

Lewis, SW .

ANALYTICA CHIMICA ACTA, 1999, 378 (1-3) :1-41

← 1 2 3 →