Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice

被引：13

作者：

Niwa, Satoru ^{[1
]}

Urtz, Nicole ^{[1
]}

Baumruker, Thomas ^{[1
]}

Billich, Andreas ^{[1
]}

Bornancin, Frederic ^{[1
]}

机构：

[1] Novartis Inst BioMed Res, A-1235 Vienna, Austria

来源：

LIPIDS IN HEALTH AND DISEASE | 2010年 / 9卷

关键词：

CYTOSOLIC PHOSPHOLIPASE A(2); LEUKEMIA HL-60 CELLS; 1-PHOSPHATE; SPHINGOMYELIN; GENERATION; INDUCTION; ANTIGEN;

D O I：

10.1186/1476-511X-9-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Ceramide kinase (CERK) produces the bioactive lipid ceramide-1-phosphate (C1P) and is a key regulator of ceramide and dihydroceramide levels. It is likely that CERK and C1P play a role in inflammatory processes but the cells involved and the mechanisms used remain to be clarified. In particular, the impact of CERK on T-cell biology has not been studied so far. Here, we used Cerk(-/-) mice backcrossed with DO11.10/RAG1(-/-) mice to probe the effect of CERK ablation on T-cell activation. Levels of interleukin (IL)-2, IL-4, IL-5, IL-13, of tumor necrosis factor (TNF)-alpha, and of interferon (INF)-gamma were recorded following ovalbumin challenge in vivo and using ovalbumin-treated splenocytes ex-vivo. Absence of CERK led to a significant decrease in the production of IL-4, thus suggesting that CERK may polarize T cells towards the T(H)2 cell subtype. However, the importance of CERK to T(H)2 cell biology will have to be investigated further because in a model of asthma, which is T(H)2-cell driven, Cerk(-/-) mice responded like wild-type animals.

引用

页数：6

共 21 条

[1]

Regulation and traffic of ceramide 1-phosphate produced by ceramide kinase - Comparative analysis to glucosylceramide and sphingomyelin [J].