Selenium and GPx-1 overexpression protect mammalian cells against UV-induced DNA damage

被引:88
作者
Baliga, Manjeshwar S.
Wang, Hengbing
Zhuo, Pin
Schwartz, Jeffrey L.
Diamond, Alan M. [1 ]
机构
[1] Univ Illinois, Dept Human Nutr, Chicago, IL 60612 USA
[2] Univ Washington, Dept Radiat Oncol, Seattle, WA 98195 USA
关键词
selenium; radiation; chemoprevention; glutathione peroxidase; DNA damage;
D O I
10.1007/BF02685998
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Supplementation of the culture media of human MCF-7 breast carcinoma cells or mouse fibroblasts with low levels of selenium (30 nM) provided as sodium selenite was shown to protect these cells from ultraviolet (UV)-induced chromosome damage, as quantified by micronucleus assay. Selenium supplementation was also effective in reducing UV-induced gene mutations as measured in the lacI shuttle vector model. Protection was dependent on functional BRCA1 activity, a protein implicated in breast cancer risk and DNA damage repair. In addition, overexpression of GPx-1, a selenoprotein with antioxidant activity, also attenuated UV-induced micronuclei formation in the absence of selenium supplementation. Combining selenium supplementation with GPx-1 overexpression further reduced UV-induced micronucleus frequency. These data provide evidence that the benefits of selenium supplementation might be either through the prevention or repair of DNA damage, and they implicate at least one selenoprotein (GPx-1) in the process.
引用
收藏
页码:227 / 241
页数:15
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